These statements have not been evaluated by the Food and Drug Administration. These products are not intended to diagnose, treat, cure, or prevent any disease. For research use only.  Legal & Disclaimer

GHRH Analog

CJC-1295

CJC-1295 is a synthetic 30-amino-acid tetrasubstituted analog of growth hormone releasing hormone (GHRH). Four amino acid substitutions confer resistance to enzymatic degradation. Available in two forms — with DAC (Drug Affinity Complex) for extended half-life, or without DAC (identical to Mod GRF 1-29).

30 Amino AcidsGHRH AnalogDAC / Non-DAC

Compound Identity

CJC-1295 at a Glance

CJC-1295 is a synthetic 30-amino-acid tetrasubstituted analog of growth hormone releasing hormone (GHRH). Four amino acid substitutions confer resistance to enzymatic degradation. Available in two forms — with DAC (Drug Affinity Complex) for extended half-life, or without DAC (identical to Mod GRF 1-29).

Compound Class

Growth hormone releasing hormone (GHRH) analog

Molecular Formula (no DAC)

C₁₅₂H₂₄₉N₄₄O₄₂

Molecular Weight (no DAC)

3,367.9 Da

Molecular Weight (with DAC)

~3,647 Da (includes MPA linker)

Amino Acid Length

30 residues (Tyr-D-Ala-Asp-Ala-Ile-Phe-Thr-Gln-Ser-Tyr-Arg-Lys-Val-Leu-Ala-Gln-Leu-Ser-Ala-Arg-Lys-Leu-Leu-Gln-Asp-Ile-Leu-Ser-Arg-Lys)

Receptor Target

Growth hormone releasing hormone receptor (GHRHR) — pituitary somatotroph

Key Modifications

D-Ala-2, Gln-8, Ala-15, Leu-27 substitutions prevent DPP-IV and endopeptidase cleavage

30 aa
Length
~3.4 kDa
Molecular Weight (no DAC)
~8 days
Half-Life (with DAC)
>98%
HPLC Purity

Research Background

Published Literature

Peer-reviewed research characterizes CJC-1295's receptor pharmacology, biochemistry, and biological activity across multiple domains. Sequence Labs supplies research-grade material; all product use is subject to laboratory research protocols and applicable regulations.

🔬

GHRHR Activation

CJC-1295 binds pituitary GHRHR with affinity comparable to native GHRH, activating Gs-coupled cAMP signaling and driving GH release from somatotroph cells.

DAC Extension

The DAC (Drug Affinity Complex) form contains an N-terminal maleimidopropionic acid (MPA) group that covalently binds serum albumin lysine residues, extending circulating half-life from ~7 minutes (native GHRH) to approximately 8 days.

🧪

Pulsatile vs Sustained

Non-DAC CJC-1295 (Mod GRF 1-29) preserves the pulsatile GH release pattern. DAC CJC-1295 produces sustained GHRH receptor stimulation — a distinct pharmacological profile used in research.

IGF-1 Elevation

Published research documents sustained IGF-1 elevation for approximately 6 days following a single DAC dose, reflecting persistent GH pulse activity.

Independent research publications on CJC-1295 are indexed in the NIH PubMed database. Consult primary literature for detailed methodology, dosing, and outcomes across research contexts.

Search PubMed

Sequence Labs Supply

Available Vial Sizes

All vials lyophilized under nitrogen with silver aluminum crimp cap and ivory-label spec. Manufacture and expiration dates printed. Batch-specific COA published in the COA library.

CJC-1295 (no DAC)

5 mg

$45 / vial

Also known as Mod GRF 1-29. Standard research vial.

CJC-1295 (no DAC)

10 mg

$75 / vial

Bulk research vial. Same 4-substitution sequence.

CJC-1295 (with DAC)

10 mg

$145 / vial

Bulk DAC vial. Research-institution volume.

Quality Standards

HPLC + Mass Spec

Every batch, reverse-phase HPLC purity confirmation plus MS molecular weight identity. Chromatograms published.

ISO 17025 Lab

Analytical testing performed by ISO 17025 accredited independent laboratory. Full documentation.

Cold-Chain Shipped

Insulated packaging with -20°C gel packs. 3-day standard, overnight available.

Silver Crimp Caps

Tamper-evident silver aluminum crimp caps. Lyophilized under nitrogen. Ivory-label spec.

Reconstitution Reference

Standard Procedure

Reconstitution protocol for research-grade lyophilized CJC-1295. Adjust diluent volume to reach the working concentration required by the research protocol.

1

Prepare Materials

Remove vial from cold storage. Allow to reach room temperature (~15-20 min). Sterile syringe ready.

2

Sterilize

Swab rubber stopper of both peptide vial and bacteriostatic water vial with 70% isopropyl alcohol.

3

Draw Diluent

For a 5 mg vial with 2 mL diluent: working concentration is 2,500 mcg/mL (2.5 mg/mL). Adjust diluent volume to preferred concentration for the research protocol.

4

Add Diluent Slowly

Inject the diluent down the inner vial wall. Never spray directly onto the lyophilized cake. Do not shake.

5

Gentle Dissolution

Swirl gently for 30-60 seconds until fully clear. CJC-1295 dissolves readily in bacteriostatic water.

6

Label + Store

Label vial with reconstitution date and concentration. Refrigerate 2-8°C. Reconstituted stability: ~4 weeks refrigerated for non-DAC; ~3 weeks for DAC form.

Handling notes: The N-terminal MPA group in DAC CJC-1295 forms an irreversible thioether bond with cysteine residues on albumin in circulation. Once reconstituted, DAC CJC-1295 remains free (not albumin-bound) in the vial — albumin conjugation occurs after administration. Store DAC and non-DAC vials separately; the two forms have distinct receptor pharmacology despite identical primary sequence.

Frequently Asked Questions

CJC-1295 FAQ

What is the difference between CJC-1295 with DAC and without DAC?

Without DAC, CJC-1295 is chemically identical to Mod GRF 1-29 (the 29-30 amino acid modified GHRH sequence) with a half-life of approximately 30 minutes. With DAC (Drug Affinity Complex), an N-terminal MPA linker covalently binds circulating albumin, extending half-life to approximately 8 days.

Why the four amino acid substitutions?

Native GHRH is degraded within minutes by DPP-IV (cleaves after position 2) and endopeptidases. D-Ala-2 blocks DPP-IV cleavage. Gln-8, Ala-15, and Leu-27 stabilize against endopeptidase attack. Together they extend the base peptide half-life ~5-6 fold before DAC.

Is CJC-1295 the same as Sermorelin?

No. Sermorelin is GHRH(1-29) — the native sequence, unmodified. CJC-1295 is a tetrasubstituted GHRH(1-29) analog. Sermorelin has a much shorter half-life (~10-15 minutes) than either form of CJC-1295.

How does CJC-1295 differ from Ipamorelin?

Different mechanism. CJC-1295 is a GHRH analog binding the GHRHR receptor. Ipamorelin is a growth hormone secretagogue receptor (GHSR / ghrelin receptor) agonist. They act at different receptors and are often studied together to compare pathways.

What testing is done on each CJC-1295 batch?

HPLC purity (typical >98%), mass spectrometry (target 3,367.9 Da for no-DAC, ~3,647 Da for DAC), endotoxin (LAL), water content by Karl Fischer, residual TFA, appearance. Full COA published.

Order CJC-1295 Research Material

Batch-tested lyophilized vials with published COA. Standard 3-day cold-chain shipping $15. Overnight $30. Same-day dispatch on orders before 2pm ET.

◆ Apply for Wholesale Access