Research Tool
Peptide Reconstitution Calculator
To reconstitute a lyophilized peptide, add bacteriostatic water (BAC water) to the vial. Use this calculator to determine the volume of BAC water needed for your desired concentration.
Research Tool
Reconstitution Calculator
Enter your vial amount, desired concentration, and target aliquot size to calculate how much bacteriostatic water to add.
BAC Water to Add
—
mL of bacteriostatic water
Volume per Aliquot
—
mL per aliquot
Total Aliquots
—
aliquots per vial
Worked Example: A 5 mg BPC-157 vial reconstituted with 2.5 mL BAC water yields a concentration of 2 mg/mL (2000 mcg/mL). A 100 mcg aliquot = 0.05 mL.
This tool is provided for laboratory research preparation only. Results represent preparation volumes for in-vitro or controlled research use — not intended for human use, self-administration, or clinical dosing guidance.
Protocol Reference
How to Reconstitute Research Peptides — Step-by-Step
Reconstituting a lyophilized peptide involves adding a precise volume of bacteriostatic water to the vial. The process takes under 2 minutes.
- Allow the vial to reach room temperature (10–15 minutes out of refrigeration)
- Using a 1 mL insulin syringe, draw the desired volume of bacteriostatic water
- Insert the needle through the rubber stopper at an angle — do not introduce solvent directly onto the lyophilized powder
- Allow the water to run down the inside wall of the vial
- Gently swirl — do not shake or vortex
- Store reconstituted peptide at 2–8°C (standard refrigerator temperature)
- Use within 28 days of reconstitution for best stability
🧫
What You Need
- Bacteriostatic water (BAC water) — preferred for multi-use vials
- Sterile water — for single-use only
- Alcohol swabs (70% isopropyl)
- Insulin syringe (1mL, 28–31 gauge)
- Clean workspace surface
⚗️
Reconstitution Steps
- Swab the vial top with an alcohol swab and allow to dry
- Draw the calculated volume of BAC water into your syringe
- Introduce solvent slowly down the side of the vial — never directly onto the lyophilized powder
- Gently swirl — do not shake
- Allow to sit for 2–3 minutes until fully dissolved
- Solution should be clear — discard if cloudy or particulate
✅
Quality Checks
- Color: Should be clear to very slightly off-white
- Clarity: No visible particles or cloudiness
- Odor: Minimal to none
- If solution appears yellow, brown, or cloudy — do not use
- Label vial with date of reconstitution
Storage Reference
Research Peptide Storage Reference
Lyophilized (freeze-dried) research peptides are stable at room temperature for short-term storage but should be kept at 2–8°C for long-term stability. Reconstituted peptides require refrigeration.
| State | Short-term | Long-term | Notes |
|---|---|---|---|
| Lyophilized (dry) | Room temp, up to 4 weeks | 2–8°C, up to 24 months Recommended | Keep away from light and moisture. Store in original sealed vial. |
| Reconstituted | 2–8°C, up to 28 days | Not recommended to freeze | Use BAC water, not sterile water, for extended usability. Label with reconstitution date. |
| Frozen (lyophilized) | — | −20°C, up to 36 months | Avoid freeze-thaw cycles. Allow to fully reach room temperature before opening. |
| Reconstituted (BAC water) | 2–8°C, 4–6 weeks | — | Keep sealed. Swab top before each use. |
| Reconstituted (sterile water) | 2–8°C, 24–48 hours | — | Single-use only. Discard remainder within 24 hours. |
| NAD+ & Mitochondrial Research Compounds | Cool, dark, dry | 2–8°C, light-protected, 12 months dry | Particularly light-sensitive. Store in amber vial or foil wrap. |
Compound Reference
Compound Research Reference Cards
Quick-reference summaries for each major peptide category in the Sequence Labs catalog. All information is for research reference only.
Research Card
GLP-1 Peptides
Semaglutide · Tirzepatide · Retatrutide
- Research Class
- GLP-1 receptor agonists
- Vial Sizes
- 5 mg, 10 mg, 15 mg, 20 mg, 30 mg
- Reconstitution
- Typically 1–2 mL BAC water per 5 mg vial
- Storage
- Lyophilized: 2–8°C. Reconstituted: 2–8°C, use within 28 days
Research Card
BPC-157 & TB-500
Structural & Repair Research Compounds
- Research Class
- Structural & repair research compounds
- Vial Sizes
- 5 mg, 10 mg
- Reconstitution
- 1–2 mL BAC water per 5 mg vial yields 2.5–5 mg/mL
- Storage
- Lyophilized stable at 2–8°C. Use reconstituted within 28 days
Research Card
NAD+ & Mitochondrial Research Compounds
NAD+ Precursors · Mitopeptides · Telomerase Research
- Research Class
- NAD+ precursors, mitopeptides, telomerase research
- Vial Sizes
- 100 mg, 500 mg, 1000 mg
- Reconstitution
- Use sterile water or BAC water per protocol
- Storage
- Lyophilized at 2–8°C, stable up to 24 months. Light-sensitive — store protected
Research Card
Growth Hormone Peptides
Ipamorelin · CJC-1295 · Sermorelin
- Research Class
- GHRH analogs, GHRP compounds, GH secretagogues
- Vial Sizes
- 5 mg, 10 mg
- Reconstitution
- 1–2 mL BAC water per vial
- Storage
- Lyophilized at 2–8°C. Reconstituted: refrigerate, use within 28 days
Research Card
GHK-Cu & Aesthetic Peptides
Copper Peptides · Melanocortin · Skin Research
- Research Class
- Copper peptides, melanocortin peptides, skin research
- Vial Sizes
- 50 mg, 100 mg (GHK-Cu); 10 mg (MT-2)
- Reconstitution
- Bacteriostatic water; GHK-Cu is highly soluble
- Storage
- 2–8°C lyophilized; avoid light exposure
Research Card
Nootropic Peptides
Selank · Semax
- Research Class
- Anxiolytic and neuroprotective peptides
- Vial Sizes
- 5 mg, 10 mg
- Reconstitution
- 1–2 mL BAC water per vial
- Storage
- Lyophilized at 2–8°C. Reconstituted: refrigerate, use within 28 days
Diluent Reference
Bacteriostatic Water for Peptide Reconstitution
Bacteriostatic water (BAC water) is the preferred diluent for reconstituting lyophilized research peptides. It contains 0.9% benzyl alcohol as a preservative, which inhibits microbial growth and extends the usability of reconstituted peptides to 28 days when refrigerated.
Bacteriostatic Water (BAC Water)
- Preservative: 0.9% benzyl alcohol
- Multi-use: Safe for repeated needle entry
- Reconstituted shelf life: Up to 28 days refrigerated
- Best for: Any peptide requiring multiple aliquots over several weeks
- pH: 4.5–7.0 (compatible with most peptides)
Sterile Water (Single-Use)
- Preservative: None
- Single-use only: No antimicrobial protection after opening
- Reconstituted shelf life: 24 hours maximum when refrigerated
- Best for: Compounds used in a single research session
- Discard unused portion after 24 hours
Sequence Labs carries BAC water at $12.99 per vial.
Available individually or in multi-packs for research convenience.
View BAC Water & Supplies
Available individually or in multi-packs for research convenience.
FAQ
Frequently Asked Research Questions
What is the difference between BAC water and sterile water?
Bacteriostatic water (BAC water) contains 0.9% benzyl alcohol, which inhibits bacterial growth and allows a vial to be used multiple times over several weeks when refrigerated. Sterile water contains no preservative and should only be used for single-use reconstitution — any unused portion should be discarded within 24–48 hours.
How do I know if my peptide has degraded?
Signs of degradation include: solution turning yellow or brown, cloudiness or visible particles, unusual odor, or loss of lyophilized (powdery) appearance in the dry state. When in doubt, discard. Store all compounds according to the storage protocol above to maximize longevity.
Can I mix two peptides in the same vial?
Combining peptides in the same vial is generally not recommended for research purposes as it complicates result attribution and may affect stability. If blended compounds are needed, Sequence Labs offers pre-formulated blends (BPC+TB, Quad Combo, etc.) that are tested as combined formulations.
What syringe size should be used for reconstitution?
For reconstitution, a standard 1mL insulin syringe (28–31 gauge, 5/16″ or 8mm needle) is appropriate. For drawing reconstituted research samples, the same syringe type is typically used. The fine gauge minimizes mechanical stress on the rubber septum and helps maintain sterility.
How should lyophilized peptides be transported?
Lyophilized peptides are relatively stable at room temperature for short durations (days to a week). For travel exceeding a few days, use a small insulated pouch with an ice pack. Reconstituted peptides should not be transported without refrigeration — if travel is necessary, lyophilized form is always preferred.
Where can I request a Certificate of Analysis for my order?
All COAs are available on request. Email Team@SequenceLabs.Health with your order number and product name. We will send the corresponding third-party batch COA within 24 hours. You can also visit our COA Library for an overview of testing standards.
How long is a reconstituted GLP-1 peptide stable?
Reconstituted GLP-1 peptides (semaglutide, tirzepatide, retatrutide) reconstituted with BAC water and stored at 2–8°C are stable for up to 28 days. Do not freeze reconstituted solutions. Always label the vial with the reconstitution date and discard after 28 days for best research integrity.
What is the recommended storage for lyophilized BPC-157?
Lyophilized BPC-157 should be stored at 2–8°C away from light and moisture. At this temperature, lyophilized vials retain stability for up to 24 months. Once reconstituted with BAC water, refrigerate at 2–8°C and use within 28 days. Do not freeze the reconstituted solution.
PubMed Research Library
Peer-Reviewed Literature by Compound
Live abstracts pulled directly from NCBI PubMed — the world's largest biomedical research database. For research reference only.
Select a compound and click Search PubMed to load the latest peer-reviewed abstracts.
Research Use Only Disclaimer. All information provided on this page is strictly for research and educational reference purposes. Sequence Labs does not provide medical advice. These materials are not intended to guide, direct, or recommend any use of research compounds in or on humans or animals. All compounds supplied by Sequence Labs are for laboratory research purposes only and are not approved by the FDA for any use.